Prediction of the structure of a receptor-protein complex using a binary docking method.

To validate procedures of rational drug design, it is important to develop computational methods that predict binding sites between a protein and a ligand molecule. Many small molecules have been tested using such programs, but examination of protein-protein and peptide-protein interactions has been sparse. We were able to test such applications once the structures of both the maltose-binding protein (MBP) and the ligand-binding domain of the aspartate receptor, which binds MBP, became available. Here we predict the binding site of MBP to its receptor using a 'binary docking' technique in which two MBP octapeptide sequences containing mutations that eliminate maltose chemotaxis are independently docked to the receptor. The peptides in the docked solutions superimpose on their original positions in the structure of MBP and allow the formation of an MBP-receptor complex. The consistency of the computational and biological results supports this approach for predicting protein-protein and peptide-protein interactions.     

Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease.

Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional growth factor that has profound regulatory effects on many developmental and physiological processes. Disruption of the TGF-beta 1 gene by homologous recombination in murine embryonic stem cells enables mice to be generated that carry the disrupted allele. Animals homozygous for the mutated TGF-beta 1 allele show no gross developmental abnormalities, but about 20 days after birth they succumb to a wasting syndrome accompanied by a multifocal, mixed inflammatory cell response and tissue necrosis, leading to organ failure and death. TGF-beta 1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions.     

铝合金变形抗力的实验研究

采用恒应变速率凸轮式形变压缩试验机试验了四种铝合金材料的变形抗力 ,分析了应变率和应变速率对变形抗力的影响 ,结果表明随应变的增加 ,变形抗力以近似幂函数关系增加 ,在此基础上建立了简单、适用的变形抗力计算模型     

论刑事附带民事赔偿的实现

刑事犯罪引起的附带民事赔偿就其性质而言，与一般的民事侵权导致的民事赔偿之间并没有本质的区别。但因前者是附带于刑事案件而启动的一种民事诉讼，导致其与一般的民事诉讼在起诉、受理、审判、执行等 多方面存在很大的区别，而且这种区别在很大程度上阻碍了刑事附带民事赔偿的最终实现，侵害了刑事案件被害人（包括国家）的合法权益，损害了刑事司法的公正性和严肃性。又因目前我国法律法规对刑事附带民事赔偿问题的规定尚存在许多不完善之处，司法实践中对此类问题的处理缺乏统一的标准，致使赔偿的实现进程步骤维艰，故对附带民事赔偿问题的深入研究具有重要的理论价值和巨大的实践意义。文章仅就赔偿的性质、范围、程序以及司法实践中存在的误区及解决方法作初步探讨。     

Abnormal pattern detected in fragile-X patients by pulsed-field gel electrophoresis.

The fragile-X syndrome is the most frequent inherited form of mental retardation, with an incidence of 1 in 1,500 males. It is characterized by the presence of a fragile site at Xq27.3 induced in vitro by folate deprivation or by inhibitors of deoxynucleotide synthesis. Its mode of inheritance is unusual for an X-linked trait, with incomplete penetrance in both males and females. Some phenotypically normal males transmit the mutation to all their daughters who rarely express any symptoms, but penetrance is high in sons and daughters of these carrier women. Genetic and physical mapping of the Xq27-q28 region has confirmed that the disease locus is located at or very near the fragile site. Hypotheses proposed to account for the abnormalities in the inheritance of the disease include sequence rearrangements by meiotic recombination or a mutation that affects reactivation of an inactive X chromosome during differentiation of female germ cells. To detect such rearrangements, or methylation changes that may reflect a locally inactive X chromosome, we used pulsed-field gel analysis of DNA from fragile-X patients with probes close to the fragile-X locus. The probe Do33 (DXS465) detected abnormal patterns in fragile-X patients, but not in normal controls or in non-expressing male transmitters.     

用测量模态阻尼和振型的方法对复合材料薄平板进行无损质量检测

复合材料薄平板经热压制成后,都需要进行质量检测。本文探索用测量模态阻尼和振型的方法,对复合材料薄平板的制作质量进行无损检测,作为对其它昂贵的无损检测方法的补充。略述用振动方法进行无损检测的原理,测量薄平板阻尼的方法以及提高测量精度的措施。还讨论在测量薄平板阻尼时可能遇到的一些特殊问题(如非线性刚度、拍、模态密集等问题);以云母基复合材料薄平板作为例子,从测量其阻尼和振型的结果,对这种板的制作质量进行讨论。     

面向WTO完善无形资产会计研究

文章借鉴有关国际贯例与经验，并结合我国实际情况，提出完善目前无形资产计量与核算的方法，并充分发挥无形资产的潜在价值，以不断优化企业财务结构，提高企业竞争能力，为企业国际化经营打下坚实的基础。     

Leaning Towards Agile in 21~(st) Century

Background: During the last fifteen years there ha s been an increased drive for organisations to reduce costs. From a production po int, this has often centred on lean manufacturing and JIT waste elimination proc esses. However, in 1991, the Iaccocca Institute Bethlehem P.A commissioned a re port specifically to analyse the changing nature of the marketplace. As a result , in the following year, the Agile Manufacturing Forum was initiated and the ter m ‘agile or responsive manufacturing‘ was first intr...